Insulin ELISA(10-1113-01)

Kit format: 1 x 96 wells

Measurement range: 3 - 200 mU/L (0.13 - 8.70 µg/L)

• Calibrated against 1st International Reference Preparation 66/304 for human insulin
• No cross-reactivity to C-peptide or Proinsulin des(31-32) or split (32-33)
• Insignificant or no cross-reactivity to insulin analogs
• CE/IVD labeled on select markets

A high quality ELISA for specific measurement of human insulin in serum, plasma or cell culture media.
 

The Mercodia insulin assays have little or no cross-reactivity to insulin analogs or to rat or mouse insulin and comes with full cross-reactivity data.

 The Mercodia Insulin ELISAs are the only ELISA assays to satisfy the total error requirements in the Insulin Standardization Program.

Test characteristics

Samples: Serum, plasma, cell culture media
Sample volume: 25 µL
Assay range: 3 - 200 mU/L
Detection limit: ≤ 1 mU/L (0.043 µg/L)
Incubation: 1 h + 15 min
Detection method: absorbance

Specificity

The following cross-reactions have been tested:

C-peptide <0.01 %
Proinsulin <0.01 %
Proinsulin des (31-32) <0.5 %
Proinsulin split (32-33) <0.5 %
Proinsulin des (64-65) 98 %
Proinsulin split (65-66) 56 %
Insulin aspart 4 %
Insulin detemir n.d.
Insulin glargin 24 %
Insulin glulisine n.d.
Insulin lispro n.d.
IGF-I <0.02 %
IGF-II <0.02 %
Rat insulin 0.7 %
Mouse insulin 0.3 %
Porcine insulin 93 %
Ovine insulin 48 %
Bovine insulin 31 %

n.d.=not detected