Proinsulin ELISA(10-1118-01)

Kit format: 1 x 96 wells

Measurement range: 3.3 - 130 pmol/L (0.03 - 1.18 µg/L)

• Measures total proinsulin
• Calibrated against International Reference Reagent IRR 84/611 for human proinsulin
• Highly specific with no cross-reactivity to human insulin or C-peptide

Mercodia Proinsulin ELISA provides a method for the quantitative determination of human proinsulin in serum or plasma.

Test characteristics

Samples: Serum and plasma
Sample volume: 50 µL
Assay range: 3.3 - 130 pmol/L
Detection limit: 1.7 pmol/L
Incubation: 1 h + 1 h + 15 min
Detection method: absorbance

Specificity

The assay is highly specific with no cross-reactivity to human insulin or C-peptide.

The following cross-reactions have been tested:

Insulin < 0.03 %
C-peptide < 0.006 %
Proinsulin (Des 31-32) 95 %
Proinsulin (Split 32-33) 95 %
Proinsulin (Des 64-65) 84 %
Proinsulin (Split 65-66) 90 %
Porcine Proinsulin 4 %
Mouse Proinsulin I 3 %
Mouse Proinsulin II 16 %
Rat Proinsulin I 13 %
Rat Proinsulin II 10 %