Ultrasensitive Insulin ELISA(10-1132-01)

Kit format: 1 x 96 wells

Measurement range: 0.15 - 20 mU/L (0.0065 - 0.87 µg/L)

• Calibrated against 1st International Reference Preparation 66/304 for human insulin
• No cross-reactivity to C-peptide or Proinsulin des(31-32) or split (32-33)
• Insignificant or no cross-reactivity to insulin analogs
• CE/IVD labeled on select markets

  A high quality enzyme immunoassay for specific quantification of human insulin in serum or plasma.
   

  The Mercodia insulin assays have little or no cross-reactivity to insulin analogs, rat or mouse insulin, which enables distinguishing endogenous human insulin, both in patients undergoing treatment with insulin analogs, and in rat or mouse models.
  
   The Mercodia Insulin ELISAs are the only ELISA assays to satisfy the total error requirements in the Insulin Standardization Program.

  Test characteristics

  Samples: Serum, plasma
  Sample volume: 25 µL
  Assay range: 0.15 - 20 mU/L
  Detection limit: ≤ 0.15 mU/L (0.0065 µg/L)
  Incubation: 1 h + 30 min
  Detection method: absorbance

  Specificity

  The following cross-reactions have been tested:
  
  C-peptide <0.01 %
  Proinsulin <0.01 %
  Proinsulin des (31-32) <0.5 %
  Proinsulin split (32-33) <0.5 %
  Proinsulin des (64-65) 98 %
  Proinsulin split (65-66) 56 %
  Insulin aspart 3 %
  Insulin detemir n.d.
  Insulin glargine 12 %
  Insulin glulisine n.d.
  Insulin lispro n.d.
  IGF-I <0.02 %
  IGF-II <0.02 %
  Rat insulin 0.7 %
  Mouse insulin 0.3 %
  Porcine insulin 93 %
  Ovine insulin 48 %
  Bovine insulin 31 %
  
  n.d.=not detected